In vitro antifungal activity and phytochemical analysis of lichen Parmelia perlata (L.) Ach.
Author(s): Vikram Singh and Dharmendra Singh
Abstract: Fungal diseases like candidiasis and cryptococcosis are the commonest opportunistic infections. Since the difficulties associated with the management of antifungal infections, there is an urgent need for the development of new and effective antifungal agents. In this perspective, natural products are a viable alternative source. The lichen
Parmelia perlata, commonly known as stone flower, is traditionally used in the Indian system of medicine. The aim of the present study was to evaluate
in vitro antifungal activity of petroleum ether extract of
Parmelia perlata against
Candida albicans,
Candida glabrata, Candida krusei, Candida tropicalis, and
Cryptococcus neoformans and its phytochemical analysis
. The anti-fungal activity of the extract of
Parmelia perlata was determined by measuring the Minimal Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) by microbroth dilution method as per the CLSI (NCCLS M27-A2) guidelines. The extract of
Parmelia perlata showed the MIC and MFC against
Candida species between 600±28µg/ml to 690±16µg/ml and between 750±90µg/ml to 820±45µg/ml, respectively. In addition, the respective values against
Cryptococcus neoformans were 550±17µg/ml and 690±34µg/ml, respectively
. Further,
Parmelia perlata has yielded an acid named- (+)-6-deacetyl-9b-carbmethoxy-9b-dimethylusnic acid and, a quinine named -6-acetyl-11-carbmethoxy-1,4,7,10-tetrahydroxy-2,8-dimethyl naphthacene-5,12-quinone, which were identified on the basis of spectroscopic studies as 2-acetyl-9b-carbmethoxy-7,9-dihydroxy-8-methyl-1,3(2H,9bH)-dibenzofurandione and naphthacene-5,12-quinone derivative, along with four well known compounds-tridecyl myristate, 3-ketooleanane, arachidyl alcohol and (+) usnic acid, respectively. The results of this study showed that
Parmelia perlata is a promising antifungal agent.
DOI: 10.33545/27072827.2020.v1.i1a.51Pages: 59-67 | Views: 504 | Downloads: 239Download Full Article: Click HereHow to cite this article:
Vikram Singh, Dharmendra Singh.
In vitro antifungal activity and phytochemical analysis of lichen Parmelia perlata (L.) Ach.. Int J Pharmacognosy Life Sci 2020;1(1):59-67. DOI:
10.33545/27072827.2020.v1.i1a.51
